Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Protein A purified
Western blot (2 µg/ml)
Immunoprecipitation (12.5 µg/ml)
Specificity and Use
A 19 residue synthetic peptide KKDVVIQDDDVESTMVEK(C) based on the human PKC alpha (residues 371-388) with a cysteine (C) residue added and the peptide coupled to KLH. The cysteine residue at position 381 of the original sequence was substituted with a ser. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (94%).
This immunoaffinity purified detects ~67-83kD proteins, corresponding to the apparent molecular masses of the different isoforms of PKC on SDS-PAGE immunoblots. This antibody is pan-specific and was designed to recognize all four cPKC isoforms (alpha, beta, gamma and delta). Species cross-reactivity: Human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, porcine, rabbit and sheep.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot (ECL): 2 ug/ml will detect cPKC in 20 ug of rat brain tissue extract. Immunoprecipitation: 12.5 ug/ml. Positive control: Mouse Brain Tissue Extract and Rat Brain Tissue Extract.