Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
PIP4K2A Antibody, PI(5)P 4-kinase type II alpha Antibody, PIP5KII-alpha Antibody, PIP5KIII Antibody, PIPK Antibody, PtdIns(4)P-5-kinase B isoform Antibody, PtdIns(4)P-5-kinase C isoform Antibody, PI5P4KA Antibody, PIP4KII-alpha Antibody, PIP5K2 Antibody, PIP5KIIalpha Antibody, PIP5K2A Antibody, PIP5KIIA Antibody
Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PIP4K2A monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIP4K2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIP4K2A.
Flow cytometry of Jurkat cells, using anti-PIP4K2A antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-PIP4K2A antibody (Red), compared to a nonspecific negative control antibody (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIP4K2A antibody, and then analyzed by flow cytometry.