Human PIN1

Rabbit

Human, Monkey, Bovine, Dog (tested or 100% immunogen sequence identity)

Monkey, Bovine, Dog (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.

A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:3000)
• Immunoprecipitation (1:100)
• ELISA (1:2500 - 1:10000)

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kD in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13526 NCBI: NM_006221 NP_006212.1