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Anti-PIM3 / PIM-3 Antibody (aa259-288) IHC-plus™ LS-B3461

Note: This antibody replaces LS-C99049

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Orders@LSBio.com
 

Most Popular PIM3 / PIM-3 Antibodies

Anti-PIM3 / PIM-3 Antibody (aa259-288) IHC-plus™ LS-B3461
Rabbit Polyclonal to Human PIM3 / PIM-3
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PIM3 / PIM-3 Antibody (C-Terminus, Biotin) LS-C280965
Rabbit Polyclonal (IgG) to Human PIM3 / PIM-3
Human
IHC, Western blot, ELISA
Biotin Conjugated
Anti-PIM3 / PIM-3 Antibody (C-Terminus, FITC) LS-C280966
Rabbit Polyclonal (IgG) to Human PIM3 / PIM-3
Human
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PIM3 / PIM-3
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human PIM3 / PIM-3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)

Specificity and Use

PIM3 / PIM-3 antibody was raised against kLH-conjugated synthetic peptide between amino acids 259-288 from C-terminal region of human PIM3.
LS-E8738 - Lyophilized - 100 µg - $145.00
aa259-288
Immunohistochemistry: LS-B3461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3461 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About PIM3 / PIM-3

Q86V86 SK200D SK200P

PIM3 Antibody, KID1 Antibody, Pim-3 oncogene Antibody, Serine/threonine kinase Pim-3 Antibody, Pim-3 Antibody

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity.

Immunohistochemistry

Immunohistochemistry
Anti-PIM3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3461 concentration 10 ug/ml.

Western blot

Western blot
The anti-PIM3 antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.

Requested From: United States
Date Requested: 12/9/2016

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