Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity.
IHC of paraffin-embedded Human colon tissue using anti-PIM2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody. (Dilution 1:50).
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PIM2 monoclonal antibody.
Anti-PIM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
Flow cytometry of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by flow cytometry.