Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti- mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti- mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti- mouse monoclonal antibody. (Dilution 1:50).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PIM2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
HEK293T cells transfected with either pCMV6-ENTRY PIM2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-PIM2 mouse monoclonal, and then analyzed by flow cytometry.