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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIK3R4 Antibody (aa360‑409) LS‑C111601
PIK3R4 antibody LS-C111601 is an unconjugated rabbit polyclonal antibody to PIK3R4 from human, mouse, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C111601-50
50 µg
Unavailable

Popular PIK3R4 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Applications: IHC, IHC - Paraffin, Western blot
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Product Description

PIK3R4 antibody LS-C111601 is an unconjugated rabbit polyclonal antibody to PIK3R4 from human, mouse, bat and other species. Validated for WB.
About PIK3R4
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Q99570 Y08991 CAA70176.1

PIK3R4 Antibody, Adaptor protein p150 Antibody, p150 Antibody, PI3-kinase p150 subunit Antibody, VPS15 Antibody


Specifications

Target
Human PIK3R4
Host
Rabbit
Reactivity
Human, Mouse, Bat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PIK3R4 antibody was raised against synthetic peptide located between aa360-409 of human PIK3R4 (Q99570, NP_055417). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Panda, Dog, Bat, Horse, Guinea pig (100%); Marmoset, Rabbit (92%); Rat (91%); Orangutan, Bovine (85%).
Blocking Peptide
LS-E19888 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111601. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa360-409
Specificity
Human PIK3R4 / p150
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PIK3R4 / p150 antibody LS-C111601 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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