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Anti-PIK3R1 / p85 Alpha Antibody (clone 6G10) IHC-plus™ LS-B8947

Note: This antibody replaces LS-C169116


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

Other PIK3R1 / p85 Alpha Products

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Mouse Monoclonal [clone 6G10] (IgG1) to Human PIK3R1 / p85 Alpha
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA


Human PIK3R1 / p85 Alpha
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6G10]


IHC - Paraffin (1:200), ICC (1:100), Immunofluorescence, Western blot (1:500 - 1:2000), Flow Cytometry (1:200 - 1:400), ELISA (1:10000)

Specificity and Use

PIK3R1 / p85 Alpha antibody was raised against purified recombinant fragment of human PIK3R1 (aa159-388) expressed in E. coli.
Human PIK3R1 / p85 Alpha


PBS, pH 7.2, 0.05% sodium azide
For long term storage -20°C is recommended.
For research use only.

About PIK3R1 / p85 Alpha

P27986 M61906 P27986

PIK3R1 Antibody, p85 Antibody, p85 Alpha Antibody, p85-ALPHA Antibody, PI3K regulatory subunit alpha Antibody, PI3-kinase subunit p85-alpha Antibody, GRB1 Antibody

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.


Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.


Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 11/28/2015

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