Human PIK3R1 / p85 Alpha
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
Recombinant human p85 alpha PI 3- Kinase expressed in E. coli.
Recognizes human p85 alpha subunit of PI 3- Kinase. Does not recognize p85 beta subunit. Species cross-reactivity: mouse and rat.
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot Analysis: 0.5-2 ug/ml detected PI 3-Kinase in RIPA lysates from A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PI 3- Kinase (0.5 mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Use 10 ug/ml. Immunoprecipitation: 4 ug.
PBS, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.