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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIK3R1 / p85 Alpha Antibody LS‑C26063
Antibody
P85 Alpha antibody LS-C26063 is an unconjugated mouse monoclonal antibody to p85 Alpha (PIK3R1) from human, mouse and rat. Validated for ICC, IP and WB.
Catalog
Size
Price
LS-C26063-100
100 µg
Unavailable

Popular PIK3R1 / p85 Alpha Products

Western blot of p-PI3 kinase p85-(Tyr607) pAb in extracts from HeLa cells.
Species: Human
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Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) antibody.
Species: Human, Mouse, Rat
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
WB of PIK3R1 / p85 Alpha antibody. All lanes: Anti-PIK3R1 at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Rabbit, Sheep, Chicken
Applications: Western blot

Product Description

P85 Alpha antibody LS-C26063 is an unconjugated mouse monoclonal antibody to p85 Alpha (PIK3R1) from human, mouse and rat. Validated for ICC, IP and WB.
About PIK3R1 / p85 Alpha
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. P27986 M61906 P27986

PIK3R1 Antibody, p85 Antibody, p85 Alpha Antibody, p85-ALPHA Antibody, PI3K regulatory subunit alpha Antibody, PI3-kinase subunit p85-alpha Antibody, GRB1 Antibody


Specifications

Target
Human PIK3R1 / p85 Alpha
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Recombinant human p85 alpha PI 3- Kinase expressed in E. coli.
Specificity
Recognizes human p85 alpha subunit of PI 3- Kinase. Does not recognize p85 beta subunit. Species cross-reactivity: mouse and rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot Analysis: 0.5-2 ug/ml detected PI 3-Kinase in RIPA lysates from A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PI 3- Kinase (0.5 mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Use 10 ug/ml. Immunoprecipitation: 4 ug.
Presentation
PBS, 50% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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