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Anti-PIK3R1 / p85 Alpha Antibody (clone 6G10) IHC-plus™ LS-B8947

Note: This antibody replaces LS-C169116
Catalog Size Price
LS-B8947-50 50 µg (1 mg/ml) Unavailable

Most Popular PIK3R1 / p85 Alpha Antibodies

Anti-PIK3R1 / p85 Alpha Antibody (phospho-Tyr607) LS-C175857
Rabbit Polyclonal to Human PIK3R1 / p85 Alpha
Human
Western blot
Unconjugated
Western blot of p-PI3 kinase p85-(Tyr607) pAb in extracts from HeLa cells.
Anti-PIK3R1 / p85 Alpha Antibody (clone 6G10) IHC-plus™ LS-B8947
Mouse Monoclonal [clone 6G10] (IgG1) to Human PIK3R1 / p85 Alpha
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha Antibody (phospho-Tyr467) LS-C205296
Rabbit Polyclonal to Human PIK3R1 / p85 Alpha
Human, Mouse, Rat
Western blot
Unconjugated
Western blot of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) antibody.
Anti-PIK3R1 / p85 Alpha Antibody (aa1-30, Biotin) LS-C284577
Rabbit Polyclonal (IgG) to Human PIK3R1 / p85 Alpha
Human
IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Biotin Conjugated
Anti-PIK3R1 / p85 Alpha Antibody (aa65-80) LS-C312573
Rabbit Polyclonal to Human PIK3R1 / p85 Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Rabbit, Sheep, Chicken
Western blot
Unconjugated
WB of PIK3R1 / p85 Alpha antibody. All lanes: Anti-PIK3R1 at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD.

100% Guaranteed
Mouse Monoclonal [clone 6G10] (IgG1) to Human PIK3R1 / p85 Alpha
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human PIK3R1 / p85 Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6G10]
Unconjugated
Purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

Purified recombinant fragment of human PIK3R1 (AA: 159-388) expressed in E. Coli.
Human, Mouse

Packaging

Purified antibody in PBS containing 0.05% sodium azide
Short term: +4°C; Long term: -20°C.
For research use only.

About PIK3R1 / p85 Alpha

P27986 M61906 P27986

PIK3R1 Antibody, p85 Antibody, p85 Alpha Antibody, p85-ALPHA Antibody, PI3K regulatory subunit alpha Antibody, PI3-kinase subunit p85-alpha Antibody, GRB1 Antibody

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

Immunohistochemistry

Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence

Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 4/25/2018

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