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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIK3R1 / p85 Alpha Antibody (clone 6G10) IHC‑plus™ LS‑B8947
Note: This antibody replaces LS-C169116
P85 Alpha antibody LS-B8947 is an unconjugated mouse monoclonal antibody to human p85 Alpha (PIK3R1). Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8947-50
50 µg (1 mg/ml)
Unavailable

Popular PIK3R1 / p85 Alpha Products

Western blot of p-PI3 kinase p85-(Tyr607) pAb in extracts from HeLa cells.
Species: Human
Applications: Western blot
Western blot of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) antibody.
Species: Human, Mouse, Rat
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
WB of PIK3R1 / p85 Alpha antibody. All lanes: Anti-PIK3R1 at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Rabbit, Sheep, Chicken
Applications: Western blot

Product Description

P85 Alpha antibody LS-B8947 is an unconjugated mouse monoclonal antibody to human p85 Alpha (PIK3R1). Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About PIK3R1 / p85 Alpha
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. P27986 M61906 P27986

PIK3R1 Antibody, p85 Antibody, p85 Alpha Antibody, p85-ALPHA Antibody, PI3K regulatory subunit alpha Antibody, PI3-kinase subunit p85-alpha Antibody, GRB1 Antibody


Specifications

Target
Human PIK3R1 / p85 Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6G10]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:100)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human PIK3R1 (AA: 159-388) expressed in E. Coli.
Specificity
Human, Mouse
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence

Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence

Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence

Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence

Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using PIK3R1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)
Western blot using PIK3R1 monoclonal antibody against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/25/2018

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