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Anti-PIK3R1 / p85 Alpha Antibody (clone 3C11) LS-C139105

Catalog	Size	Price
LS-C139105-100	100 µg	Unavailable

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Antibody:	Mouse Monoclonal [clone 3C11] (IgG1,k) to Human PIK3R1 / p85 Alpha
Reactivity:	Human, Mouse, Rat
Application:	Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human PIK3R1 / p85 Alpha
Host:	Mouse
Reactivity:	Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality:	IgG1,k Monoclonal [3C11]
Conjugations:	Unconjugated
Purification:	Purified from ascites by Protein A
Modifications:	Unmodified

Applications

Approved:

Western blot
ELISA

Specificity and Use

Immunogen:	PIK3R1 / p85 Alpha antibody was raised against pIK3R1 (AAH30815, 1 a.a. ~ 455 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity:	Human PIK3R1 / p85 Alpha
Usage:	Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Cell lysate, Recombinant protein.

Packaging

Presentation:	PBS, pH 7.2
Storage:	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Restrictions:	For research use only.

About PIK3R1 / p85 Alpha

Uniprot: P27986 NCBI: M61906 P27986

Synonyms: PIK3R1 Antibody, p85 Antibody, p85 Alpha Antibody, p85-ALPHA Antibody, PI3K regulatory subunit alpha Antibody, PI3-kinase subunit p85-alpha Antibody, GRB1 Antibody

Description: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

(More About PIK3R1 / p85 Alpha)

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Western blot

PIK3R1 monoclonal antibody (M03), clone 3C11. Western blot of PIK3R1 expression in Raw 264.7.

Western blot

PIK3R1 monoclonal antibody (M03), clone 3C11. Western blot of PIK3R1 expression in NIH/3T3.

Western blot

PIK3R1 monoclonal antibody (M03), clone 3C11. Western blot of PIK3R1 expression in HeLa.

Western blot

PIK3R1 monoclonal antibody (M03), clone 3C11. Western blot of PIK3R1 expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PIK3R1 is approximately 3 ng/ml as a capture antibody.

Requested From:
Date Requested: 6/27/2017

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