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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIK3CA / PI3K Alpha Antibody (clone 3G3) LS‑C197904
PI3K Alpha antibody LS-C197904 is an unconjugated mouse monoclonal antibody to human PI3K Alpha (PIK3CA). Validated for ELISA.
Catalog
Size
Price
LS-C197904-100
100 µg
Unavailable

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Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

PI3K Alpha antibody LS-C197904 is an unconjugated mouse monoclonal antibody to human PI3K Alpha (PIK3CA). Validated for ELISA.
About PIK3CA / PI3K Alpha
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. P42336 NM_006218 NP_006209.2

PIK3CA Antibody, MCAP Antibody, p110-alpha Antibody, p110alpha Antibody, PI3K Antibody, PI3-kinase p110 subunit alpha Antibody, PI3-kinase subunit alpha Antibody, PI3Kalpha Antibody, MCM Antibody, MCMTC Antibody, PI3K-alpha Antibody, PtdIns-3-kinase subunit alpha Antibody, CLOVE Antibody


Specifications

Target
Human PIK3CA / PI3K Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3G3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • ELISA
Immunogen
PIK3CA (NP_006209, aa959-1068 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PIK3CA / PI3K
Usage
Sandwich ELISA: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PIK3CA is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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