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PICALM / CALM Antibody (aa438‑487) LS‑C146567
CALM antibody LS-C146567 is an unconjugated rabbit polyclonal antibody to CALM (PICALM) from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C146567-50
50 µg (1 mg/ml)
Unavailable

Popular PICALM / CALM Products

Anti-PICALM antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5065 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PICALM / CALM antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10874 concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
All lanes: Anti-PICALM Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: HeLa whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lane 5: rat brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-PICALM Antibody (C-Term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CALM antibody LS-C146567 is an unconjugated rabbit polyclonal antibody to CALM (PICALM) from human, rat, bat and other species. Validated for WB.
About PICALM / CALM
PICALM / CALM is a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Q13492 NM_007166 NP_009097.2

PICALM Antibody, CALM Antibody, LAP Antibody, CLTH Antibody


Specifications

Target
Human PICALM / CALM
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
PICALM / CALM antibody was raised against synthetic peptide located between aa438-487 of human PICALM (E9PN05, NP_001193876). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%); Mouse, Zebra finch (92%); Chicken, Zebrafish (85%); Lizard, Salmon (84%).
Epitope
aa438-487
Specificity
Human PICALM
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Reconstitute with 50ul sterile ddH2O.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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