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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74883-50 | 50 µg | $658 |
Monoclonal Mouse Phosphotyrosine Antibody (IHC, WB) LS‑C74883
Monoclonal Mouse Phosphotyrosine Antibody (IHC, WB) LS‑C74883
Antibody:
Phosphotyrosine Mouse Monoclonal Antibody
Application:
IHC, ICC, WB, IP
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Phosphotyrosine Mouse Monoclonal Antibody
Application:
IHC, ICC, WB, IP
Format:
Unconjugated, Unmodified
Specifications
Description
Phosphotyrosine antibody LS-C74883 is an unconjugated mouse monoclonal antibody to Phosphotyrosine. Validated for ICC, IHC, IP and WB.
Host
Mouse
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Phosphotyramine (KLH coupled).
Specificity
Recognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine.
Applications
- IHC
- ICC
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
IP: 2-4ug immunoprecipitates quantitatively the phosphotyrosine-containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2mM sodium orthovanadate to the lysis buffer. WB: Detects tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
