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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74922-100 | 100 µg | $890 |
Monoclonal Mouse Phosphotyrosine Antibody (WB) LS‑C74922
Monoclonal Mouse Phosphotyrosine Antibody (WB) LS‑C74922
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Phosphotyrosine antibody LS-C74922 is an unconjugated mouse monoclonal antibody to Phosphotyrosine. Validated for IP and WB.
Host
Mouse
Clonality
IgG2b,k
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Phosphotyramine (KLH coupled).
Specificity
Recognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
IP: 2-4ug. Immunoprecipitates quantitatively the phosphotyrosine-containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2mM sodium orthovanadate to the lysis buffer. WB: Detects tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
Presentation
0.01 M Tris-HCl, pH 8.0, 0.15 M NaCl, 0.02% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
