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Anti-Phosphotyrosine Antibody LS-C74920


Wt. Vol. Conc. Price
100 µg - - $560
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Phosphotyrosine Antibodies

Anti-Phosphotyrosine Antibody LS-C74941
Mouse Monoclonal (IgG1)
ICC, Western blot, Immunoprecipitation, ELISA

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
Western blot, ELISA


IgG Polyclonal
Affinity purified


  • Western blot (1:5000 - 1:30000)
  • ELISA (1:30000)

Specificity and Use

Phosphotyrosine antibody was raised against a random amino acid polymer containing phosphotyrosine (KLH coupled).
Specific for phosphotyrosine. Does not cross-react with phosphoserine, phosphothreonine, serine, threonine or tyrosine as confirmed by ELISA and Western Blot using phosphorylated and unphosphosphorylated protein. Species cross-reactivity: All species.
Suitable for use in Western Blot, Slot Blot and ELISA. Western Blot: 1:5000-1:30000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1000 the antibody was easily able to detect 10 ng (0.2pmol of a phosphorylated peptide) using chromogenic (BCIP/NBT) development. Slot Blot: 1:10000. ELISA: 1:30000-1;100000 for detection of 1 ug/well of phosphotyrosine-containing substrate. Positive control: EGF-stimulated A431 cells containing high levels of phosphorylated EGF receptor.


0.05 M Tris, 0.15 M sodium chloride, pH 7.2, 0.02% sodium azide.
For research use only.

About Phosphotyrosine

Some of the tyrosine residues in protein can be modified (at the hydroxyl group) with a phosphate group (phosphorylated) by protein kinases. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies.

Requested From: United States
Date Requested: 10/22/2016

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