Synthetic Phosphotyrosine

Mouse

Synthetic (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

PY20

Unconjugated

Affinity purified

Unmodified

Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

• Immunofluorescence
• Western blot (2 µg/ml)
• ELISA

Western Blot: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1 mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

BBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.