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Anti-Phosphotyrosine Antibody (clone PY20) LS-C57995


Wt. Vol. Conc. Price
500 µg - 0.5 mg/ml $275
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Phosphotyrosine Antibodies

Anti-Phosphotyrosine Antibody LS-C74941
Mouse Monoclonal (IgG1)
ICC, Western blot, Immunoprecipitation, ELISA

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone PY20] (IgG2b)
Immunofluorescence, Western blot, ELISA


IgG2b Monoclonal [PY20]
Affinity purified


  • Immunofluorescence
  • Western blot (2 µg/ml)

Specificity and Use

Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Western Blot: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1 mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.


100 mM borate buffered saline, pH 8.0, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Phosphotyrosine

Some of the tyrosine residues in protein can be modified (at the hydroxyl group) with a phosphate group (phosphorylated) by protein kinases. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies.

Requested From: United States
Date Requested: 10/28/2016

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