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Anti-Phosphothreonine Antibody LS-C74910

Catalog Size Price
LS-C74910-100 100 µl Unavailable

Most Popular Phosphothreonine Antibodies

Anti-Phosphothreonine Antibody (clone 18F6) LS-C60053
Mouse Monoclonal [clone 18F6]
Western blot, ELISA
Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Anti-Phosphothreonine Antibody LS-C74909
Mouse Monoclonal (IgG2a,k)
Western blot, Immunoprecipitation, ELISA
Anti-Phosphothreonine Antibody LS-C74913
Mouse Monoclonal (IgG1,k)
Western blot, Immunoprecipitation, ELISA
Anti-Phosphothreonine Antibody LS-C74915
Mouse Monoclonal (IgM)
Western blot, Immunoprecipitation, ELISA
Anti-Phosphothreonine Antibody LS-C76374
Rabbit Polyclonal (IgG)
Western blot, Immunoprecipitation, ELISA

100% Guaranteed
Mouse Monoclonal (IgG1,k)
Western blot, ELISA


IgG1,k Monoclonal


  • Western blot (1:500)
  • ELISA (1:1000)

Specificity and Use

Phosphothreonine conjugated to KLH.
Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal cross-reactivity is observed against phosphoserine or phosphotyrosine lysates.
Suitable for use in ELISA and Western Blot. ELISA: 1:1000 to 1:5000, assayed against phosphothreonine conjugated BSA using IgG, H&L (HRP) Pab Gt xMo for detection. Western Blot: 1:500.


0.01% sodium azide, 40% glycerol. No buffer or stabilizers added. No stabilizing proteins added.
For research use only.

About Phosphothreonine

Requested From: 
Date Requested: 5/22/2018

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