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Anti-Phosphothreonine Antibody LS-C74909

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular Phosphothreonine Antibodies

Anti-Phosphothreonine Antibody LS-C74909
Mouse Monoclonal (IgG2a,k)
Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a,k)
Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Phosphothreonine
Mouse
IgG2a,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.
Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 ug/100 ul/well) to an anti-phosphothreonine coated microplate (0.5 ug/100 ul/well) is inhibited by 95% with the addition of 2-10 ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/ml. Species cross-reactivity: species independent.
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. ELISA: 0.5 ug/ml (kinase assay). Western Blot: 2 ug/ml versus mouse brain lysate. Incubate at RT for 4 hours.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Phosphothreonine


Requested From: United States
Date Requested: 2/25/2017

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