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Anti-Phosphothreonine Antibody (clone 18F6) LS-C60053

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular Phosphothreonine Antibodies

Anti-Phosphothreonine Antibody LS-C74909
Mouse Polyclonal (IgG2a,k)
Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 18F6]
Western blot, ELISA
Unconjugated

Details

Phosphothreonine
Mouse
Monoclonal [18F6]
Unconjugated
Ascites
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

Phosphothreonine conjugated KLH.
Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine
This monoclonal antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.

Packaging

0.01% sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

About Phosphothreonine


Western blot

Western blot
Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Anti-Phosphothreonine Antibody - ELISA. ELISA results of Mab anti-phosphothreonine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate were used for detection.

Requested From: United States
Date Requested: 12/7/2016

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