Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Gel filtration
Immunogen
Phosphoserine containing peptides.
Specificity
Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine. Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species cross-reactivity: human, mouse, rat and canine.
Applications
- Western blot (1 µg/ml)
- Immunoprecipitation (1 - 10 µg/10E6 cells)
- ELISA (0.05 µg/ml)
Usage
IP: Vanadate treated A431 cells. WB: For HRPO/ECL detection. Recommended blocking buffer: BPPT:3%BSA, 1% PEG 4000, 1% PVP, 0.1% Tween 20, 2X PBS. Note: DO NOT USE Milk/Casein for Blocking and dilution! Replace with BSA
Presentation
PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee