Clonality
IgM,k
Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Immunogen
Phosphoserine containing peptides.
Specificity
Species cross-reactivity: Human, Mouse, Rat, Dog. Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine. Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence.
Applications
- Western blot (1 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Immunoprecipitation: 1-10 ug per 1E6 pervanadate-treated A431 cells. Western Blot: 1 ug/ml with HRP/ECL detection. BSA/Tween 20 based blocking buffer. Do not use milk/casein for blocking and dilution!
Presentation
PBS, 0.09% Sodium Azide, Sucrose, PEG
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee