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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5457-50 | 50 µg | $460 |
![PHGDH Antibody - Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~10 ug/ml].](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-phgdh-antibody-clone-4a3-1d6-ls-b5457/52693_19132.jpg)
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IHC‑plus™ Monoclonal Mouse anti‑Human PHGDH Antibody (clone 4A3‑1D6, IHC, IF, WB) LS‑B5457
IHC‑plus™ Monoclonal Mouse anti‑Human PHGDH Antibody (clone 4A3‑1D6, IHC, IF, WB) LS‑B5457
Note: This antibody replaces LS-C133714
Antibody:
PHGDH Mouse anti-Human Monoclonal (4A3-1D6) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PHGDH Mouse anti-Human Monoclonal (4A3-1D6) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PHGDH antibody LS-B5457 is an unconjugated mouse monoclonal antibody to human PHGDH. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PHGDH
Synonyms
PHGDH | 3-PGDH | 3PGDH | PGDH | Phosphoglycerate dehydrogenase | PDG | PGAD | PGDH3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
4A3-1D6
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
PHGDH (AAH11262, 1 aa ~ 534 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PHGDH
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (1 - 10 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PHGDH
Validation
Anti-PHGDH antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PHGDH antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PHGDH Antibody (clone 4A3-1D6) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B5457 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. Gromova I, Gromov P, Honma N, Kumar S, Rimm D, Talman ML, Wielenga VT, Moreira JM. Molecular oncology. 2015 9:1636-54. (WB; Human)
Customer Reviews (0)
Featured Products
Reactivity:
Human
Range:
0.156-10 ng/ml
Reactivity:
Pig
Range:
78-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
