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Anti-PHGDH Antibody (aa362-411) LS-C81937

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PHGDH Antibodies

Anti-PHGDH Antibody (aa362-411) LS-C81937
Rabbit Polyclonal (IgG) to Human PHGDH
Human, Monkey, Mouse, Rat, Hamster
Western blot
Unconjugated
Western blot Image
Anti-PHGDH Antibody (clone 4A3-1D6) IHC-plus™ LS-B5457
Mouse Monoclonal [clone 4A3-1D6] (IgG1,k) to Human PHGDH
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PHGDH
Human, Monkey, Mouse, Rat, Hamster
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PHGDH
Rabbit
Human, Monkey, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Dog, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

PHGDH antibody was raised against synthetic peptide located between aa362-411 of human PHGDH (O08651, NP_006614). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%); Dog, Rabbit (92%); Galago (90%); Elephant, Pig (85%).
aa362-411
Human PHGDH
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PHGDH

O43175 NM_006623 NP_006614.2

PHGDH Antibody, 3-PGDH Antibody, 3PGDH Antibody, PGDH Antibody, Phosphoglycerate dehydrogenase Antibody, PDG Antibody, PGAD Antibody, PGDH3 Antibody

PHGDH encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Western blot

Western blot
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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