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Anti-PHF2 Antibody IHC-plus™ LS-B172

Note: This antibody replaces LS-C2842

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PHF2 Antibodies

Anti-PHF2 Antibody IHC-plus™ LS-B172
Rabbit Polyclonal
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PHF2 Antibody (aa936-985) LS-C109733
Rabbit Polyclonal to Human PHF2
Human, Rabbit
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal
Human
IHC - Paraffin, Western blot
Unconjugated

Details

PHF2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Purified IgG
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:500 - 1:1000)

Specificity and Use

PHF2 antibody was raised against internal peptide residues made to the 120 kD PHF protein
Reacts with residues 70-82 [KHGPGPTPDVKRVC] of the 120 kD PHF protein.
Immunohistochemistry: LS-B172 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B172 was determined to be 20 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PHF2

O75151 NM_005392 NP_005383.3

PHF2 Antibody, GRC5 Antibody, JHDM1E Antibody, KIAA0662 Antibody, PHD finger protein 2 Antibody, CENP-35 Antibody, Centromere protein 35 Antibody

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes.

Immunohistochemistry

Immunohistochemistry
Anti-PHF2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B172 concentration 20 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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