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Anti-PHEX / PEX Antibody (C-Terminus) LS-C9583

Catalog Size Price
LS-C9583-50 50 µg (1 mg/ml) Unavailable

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Immunofluorescence staining of HepG2 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Rabbit Polyclonal (IgG) to Rat PHEX / PEX
Western blot, ELISA


Rat (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • Western blot (1 - 10 µg/ml)
  • ELISA (1:10000 - 1:100000)

Specificity and Use

PHEX / PEX antibody was raised against synthetic peptide sequence consisting of 17aa within the extracellular, C-terminus of rat PHEX (1) (KLH coupled).
Recognizes PHEX. No significant sequence homology of PHEX11-P exists with NEP, NEPLs or other M13-family proteases. Species sequence Homology: Mouse: 94%; human: 88%.
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.


PBS, 0.1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PHEX / PEX

P78562 NM_000444 NP_000435.3

PHEX Antibody, HYP Antibody, HYP1 Antibody, LXHR Antibody, HPDR Antibody, PEX Antibody, XLH Antibody, HPDR1 Antibody

PHEX / PEX is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants.

Requested From: 
Date Requested: 5/22/2018

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