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Anti-PGM3 Antibody (clone 1E2-1B12) LS-C133376

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PGM3 Antibodies

Anti-PGM3 Antibody (aa237-263, AP) LS-C238956
Rabbit Polyclonal (IgG) to Human PGM3
Human
Western blot, ELISA
AP Conjugated
Anti-PGM3 Antibody (aa237-263, Biotin) LS-C238958
Rabbit Polyclonal (IgG) to Human PGM3
Human
Western blot, ELISA
Biotin Conjugated
Anti-PGM3 Antibody (aa237-263, Azide-free, HRP) LS-C248457
Rabbit Polyclonal (IgG) to Human PGM3
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1E2-1B12] (IgG1,k) to Human PGM3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PGM3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1E2-1B12]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

PGM3 antibody was raised against pGM3 (AAH01258, 1 a.a. ~ 543 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PGM3
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PGM3

O95394 NM_015599 NP_056414.1

PGM3 Antibody, AGM1 Antibody, DKFZP434B187 Antibody, PAGM Antibody, Phosphoglucomutase-3 Antibody, PGM 3 Antibody, Phosphoglucomutase 3 Antibody

PGM3 is a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2 ug/ml].

Western blot

Western blot
PGM3 monoclonal antibody (M01), clone 1E2-1B12 Western blot of PGM3 expression in HeLa.

Western blot

Western blot
Western blot of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PGM3 is approximately 3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody, clone 1E2-1B12. GAPDH ( 36.1 kD ) used as specific.

Requested From: United States
Date Requested: 12/4/2016

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