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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PGAM2 Antibody (clone 2F5) LS‑C173246
PGAM2 antibody LS-C173246 is an unconjugated mouse monoclonal antibody to PGAM2 from human, mouse, rat and other species. Validated for IF and WB.
Catalog
Size
Price
LS-C173246-100
100 µl (0.72 mg/ml)
Unavailable

Popular PGAM2 Products

Antibody
Species: Rabbit
Applications: IHC, Immunofluorescence, Western blot, ELISA
IHC of paraffin-embedded Human Kidney tissue using anti-PGAM2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-PGAM2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

PGAM2 antibody LS-C173246 is an unconjugated mouse monoclonal antibody to PGAM2 from human, mouse, rat and other species. Validated for IF and WB.
About PGAM2
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. P15259 NM_000290 NP_000281.2

PGAM2 Antibody, BPG-dependent PGAM 2 Antibody, GSD10 Antibody, PGAMM Antibody, PGAM-M Antibody, Phosphoglycerate mutase 2 Antibody


Specifications

Target
Human PGAM2
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [2F5]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Immunogen
PGAM2 antibody was raised against full length human recombinant of human PGAM2(NP_000281) produced in HEK293 cell.
Specificity
Human PGAM2
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Immunofluorescence

Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Immunofluorescence

Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Immunofluorescence

Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.


Requested From: 
Date Requested: 6/22/2018

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