(tested or 100% immunogen sequence identity)
Dog, Pig (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 - 3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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PFKFB2 antibody was raised against synthetic peptide SEQNNNSYETKTP-C from the N-terminus of human PFKFB2 (NP_006203.2; NP_001018063.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Orangutan, Dog, Pig (92%); Bovine (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6492. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human PFKFB2. This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.