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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PFKFB2 Antibody (aa7‑19) IHC‑plus™ LS‑B6492
Note: This antibody replaces LS-C112876
PFKFB2 antibody LS-B6492 is an unconjugated goat polyclonal antibody to PFKFB2 from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6492-50
50 µg (0.5 mg/ml)
Unavailable

Popular PFKFB2 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of PFKFB2 (pS483) expression in HeLa H2O2-treated (A); SP2/0 H2O2-treated (B); H9C2 H2O2-treated (C) whole cell lysates.
Species: Human, Mouse, Rat, Bovine
Applications: Western blot
Western blot of Phospho-PFK-2 car (S483) antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Western blot of PFK-2 car antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

PFKFB2 antibody LS-B6492 is an unconjugated goat polyclonal antibody to PFKFB2 from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About PFKFB2
PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. O60825 NM_006212 NP_006203.2

PFKFB2 Antibody, 6PF-2-K/Fru-2,6-P2ase 2 Antibody, PFK-2/FBPase-2 Antibody, PFK/FBPase 2 Antibody, PFKFB, cardiac Antibody


Specifications

Target
Human PFKFB2
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Dog, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PFKFB2 antibody was raised against synthetic peptide SEQNNNSYETKTP-C from the N-terminus of human PFKFB2 (NP_006203.2; NP_001018063.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Orangutan, Dog, Pig (92%); Bovine (85%).
Blocking Peptide
LS-E27177 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6492. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa7-19
Specificity
Human PFKFB2. This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.
Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.
Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.
Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.
Anti-PFKFB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6492 concentration 2.5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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