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Anti-Peripherin Antibody LS-C204575

Ordering

Wt. Vol. Conc. Price
- 100 µl - Unavailable

Related Products

48 Peripherin Antibodies

Most Popular Peripherin Antibodies

Anti-Peripherin Antibody LS-C2839
Rabbit Polyclonal to Rat Peripherin
Rat, Human, Mouse, Mammal
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image
Anti-Peripherin Antibody (N-Terminus) LS-C26027
Rabbit Polyclonal (IgG) to Rat Peripherin
Rat, Human, Mouse, Bovine, Pig
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-Peripherin Antibody LS-C61831
Chicken Polyclonal to Rat Peripherin
Rat, Mammal
Immunofluorescence, Western blot
Unconjugated
Western blot Image
Anti-Peripherin Antibody LS-C74256
Mouse Monoclonal to Rat Peripherin
Rat, Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Anti-Peripherin Antibody (clone 3B3) IHC-plus™ LS-B6138
Mouse Monoclonal [clone 3B3] (IgG2b,k) to Human Peripherin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human Peripherin
Human
IHC, ICC, Western blot
Unconjugated

Details

Human Peripherin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC (1:1000)
  • ICC (1:1000)
  • Western blot (1:20000)

Specificity and Use

Peripherin antibody was raised against recombinant full length peripherin purified from E. coli.
Human Peripherin
For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methods. For immunoblotting 1:20000 is recommended.

Packaging

Antiserum
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Peripherin

P41219 NM_006262 NP_006253.2

PRPH Antibody, NEF4 Antibody, Neurofilament 4 (57kD) Antibody, Peripherin Antibody, PRPH1 Antibody, Neurofilament 4 Antibody

Peripherin is a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.

Immunocytochemistry

Immunocytochemistry
Mixed neuron/glia cultures from newborn rat brain stained with MCS-7C5 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H CPCA-NF-H (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.

Western blot

Western blot
Western blot of whole rat brain stem homogenate stained with Peripherin antibody, at dilution of 1:20000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Peripherin. A lower band at ~48kDa is derived from the Peripherin molecule.

Requested From: 
Date Requested: 4/25/2017

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