Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Peripherin is a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.
Rat mixed neuron/glial cultures stained with Peripherin antibody, (green channel) and the rabbit polyclonal antibody to the neurofilament subunit alpha-internexin (red). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and alpha-internexin, the green and red signals superimpose to produce a golden yellow cell. The blue signal is a DNA stain and reveals the nuclei of neurons and non-neuronal cells.
Left: Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (lane 1), brain stem (lane 2), cerebellum (lane 3), and spinal cord (lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by NF-LMCA, NF-MMCA and MCA-NF-HMCA, spectrin/fodrin by S/FMCA and Microtubule associated protein 2 by MAP2MCA. Right: western blot of similar preparations separated on 6% SDS-PAGE and processed for immunoblotting with Peripherin antibody at a dilution of 1:10,000. A clear band running at 57kDa apparent size is seen in the spinal cord and brain stem lanes, but not in the other regions, in which peripherin is a very minor component. The position of this band on the Coomassie stained preparation is a little higher on the 8% gel and is indicated by PeriMCA in the left panel.