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Anti-Peripherin Antibody LS-C204577

Ordering

Wt. Vol. Conc. Price
- 100 µl 21.5 mg/ml $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Peripherin Antibodies

Anti-Peripherin Antibody LS-C74256
Mouse Monoclonal to Rat Peripherin
Rat, Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Anti-Peripherin Antibody (clone 3B3) IHC-plus™ LS-B6138
Mouse Monoclonal [clone 3B3] (IgG2b,k) to Human Peripherin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Human Peripherin
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human Peripherin
Chicken
Human (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:2000)
  • Immunofluorescence (1:2000)
  • Western blot (1:10000)

Specificity and Use

The IgY solution can be used at dilutions of at least 1:2000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Packaging

PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Peripherin

P41219 NM_006262 NP_006253.2

PRPH Antibody, NEF4 Antibody, Neurofilament 4 (57kD) Antibody, Peripherin Antibody, PRPH1 Antibody, Neurofilament 4 Antibody

Peripherin is a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.

Immunocytochemistry

Immunocytochemistry
Rat mixed neuron/glial cultures stained with Peripherin antibody, (green channel) and the rabbit polyclonal antibody to the neurofilament subunit alpha-internexin (red). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and alpha-internexin, the green and red signals superimpose to produce a golden yellow cell. The blue signal is a DNA stain and reveals the nuclei of neurons and non-neuronal cells.

Western blot

Western blot
Left: Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (lane 1), brain stem (lane 2), cerebellum (lane 3), and spinal cord (lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by NF-LMCA, NF-MMCA and MCA-NF-HMCA, spectrin/fodrin by S/FMCA and Microtubule associated protein 2 by MAP2MCA. Right: western blot of similar preparations separated on 6% SDS-PAGE and processed for immunoblotting with Peripherin antibody at a dilution of 1:10,000. A clear band running at 57kDa apparent size is seen in the spinal cord and brain stem lanes, but not in the other regions, in which peripherin is a very minor component. The position of this band on the Coomassie stained preparation is a little higher on the 8% gel and is indicated by PeriMCA in the left panel.

Requested From: United States
Date Requested: 12/5/2016

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