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Anti-Peripherin Antibody (clone 7C5) LS-C204573

Ordering

Wt. Vol. Conc. Price
- 500 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Peripherin Antibodies

Anti-Peripherin Antibody LS-C74256
Mouse Monoclonal to Rat Peripherin
Rat, Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Anti-Peripherin Antibody (clone 3B3) IHC-plus™ LS-B6138
Mouse Monoclonal [clone 3B3] (IgG2b,k) to Human Peripherin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7C5] (IgG1,k) to Human Peripherin
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human Peripherin
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [7C5]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • ICC (1:200)
  • Western blot (1:1000)

Specificity and Use

Peripherin antibody was raised against recombinant rat peripherin purified from E. coli.
The antibody is clean and specific for the expected 57 kDa band on Western blots. Subsequent studies indicated that 7C5 is relatively insensitive to aldehyde fixation and is superior for this purpose than 8G2, a widely used anti-peripherin monoclonal antibody. 7C5 stains peripherin in a variety of mammalian species, but gives equivocal results on chicken tissues. 7C5 has a mouse IgG1 with a kappa light chain.
For immunocytochemistry we recommend dilutions of 1:200, and for western blot 1:1000.

Packaging

Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Peripherin

P41219 NM_006262 NP_006253.2

PRPH Antibody, NEF4 Antibody, Neurofilament 4 (57kD) Antibody, Peripherin Antibody, PRPH1 Antibody, Neurofilament 4 Antibody

Peripherin is a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.

Immunocytochemistry

Immunocytochemistry
Mixed neuron/glia cultures from newborn rat brain stained with Peripherin antibody antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
 

Requested From: United States
Date Requested: 12/8/2016

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