Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3F6B3] (IgG1) to Streptococus mutans Peptidoglycan
ICC, ELISA, Dot Blot
Streptococus mutans Peptidoglycan
Streptococus mutans (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3F6B3]
Specificity and Use
Peptidoglycan antibody was raised against insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).
Suitable for use in ELISA, Dot Blot and Immunocytochemistry. ELISA: Competitive or solid phase. Immunocytochemistry: Although PG is a bacterial surface component, pretreatments, such as treatment with strong acid, for Gram-positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria, may be necessary to expose the epitopes.
Supplied as a liquid. No preservatives added.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.