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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PEPD / PROLIDASE Antibody (clone 5D5) LS‑C115594
PROLIDASE antibody LS-C115594 is an unconjugated mouse monoclonal antibody to PROLIDASE (PEPD). Validated for Flow and WB.
Catalog
Size
Price
LS-C115594-100
100 µl
Unavailable

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Product Description

PROLIDASE antibody LS-C115594 is an unconjugated mouse monoclonal antibody to PROLIDASE (PEPD). Validated for Flow and WB.
About PEPD / PROLIDASE
PEPD / PROLIDASE is a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. P12955 NM_000285 NP_000276.2

PEPD Antibody, Aminoacyl-L-proline hydrolase Antibody, Imidodipeptidase Antibody, PRD Antibody, PROLIDASE Antibody, X-Pro dipeptidase Antibody, Xaa-Pro dipeptidase Antibody, Peptidase D Antibody, Proline dipeptidase Antibody


Specifications

Target
Human PEPD / PROLIDASE
Host
Mouse
Clonality
IgG1 Monoclonal [5D5]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell
Specificity
Human PEPD
Usage
Concentration: 0.81 mg/ml (Lot dependent)
Presentation
PBS (PH 7.3), 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody, (Red), compared to a nonspecific negative control antibody, (Blue).


Requested From: 
Date Requested: 6/19/2018

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