Pig PECAM-1 / CD31
(tested or 100% immunogen sequence identity)
PECAM-1 / CD31 antibody was raised against porcine CD31/human IgGFc fusion protein.
Recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutant protein binding studies. Immunoprecipitates a protein of ~130 kD from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 100 ul of lysed whole blood or 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized, PBS, 0.09% sodium azide, 5% sucrose
100 tst Reconstitute with 1ml distilled water.
Lyophilized powder may be stored at 4°C before opening. DO NOT FREEZE! Aliquot and store at 4°C. Stable at 4°C as an undiluted liquid for 6 months. Dilute only prior to immediate use.
For research use only.
This antibody carries the LSBio 100% Guarantee.