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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PECAM-1 / CD31 Antibody (clone 2F7B2) IHC-plus™

Note: This antibody replaces LS-C41483
CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B3446-50
50 µl
$425
Description
CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PECAM-1 / CD31
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2F7B2]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PECAM-1 / CD31 antibody was raised against purified recombinant fragment of human CD31 expressed in E. Coli.
Specificity
Human
Usage
Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
About PECAM-1 / CD31
P16284 NM_000442 NP_000433.4

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Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
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IHC of rat spleen cryosection with Mouse anti-Rat CD31 (MOUSE ANTI RAT CD31).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Anti-CD31 antibody IHC of human tonsil, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Publications (1)

1
Vascular pathology in dermatomyositis and anatomic relations to myopathology. Pestronk A, Schmidt RE, Choksi R. Muscle & nerve. 2010 42:53-61. (IHC; Human) [Full Text Article] [PubMed:20544925]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunohistochemistry

Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.
Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.

Immunofluorescence

Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunohistochemistry

Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.
Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.

Immunofluorescence

Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunohistochemistry

Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.
Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.

Immunofluorescence

Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunohistochemistry

Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.
Western blot using CD31 mouse monoclonal antibody against truncated CD31 recombinant protein.

Immunofluorescence

Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Requested From: United States
Date Requested: 1/23/2019
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