CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Human PECAM-1 / CD31
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:200)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
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PECAM-1 / CD31 antibody was raised against purified recombinant fragment of human CD31 expressed in E. Coli.
Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200.
Ascitic fluid containing 0.03% sodium azide
Short term: 4°C; Long term: -20°C.
For research use only.