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Anti-PECAM-1 / CD31 Antibody (clone ER-MP12, Biotin) LS-C96364

Ordering

Wt. Vol. Conc. Price
300 µg 500 µl 0.6 mg/ml $545
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular PECAM-1 / CD31 Antibodies

Anti-PECAM-1 / CD31 Antibody IHC-plus™ LS-B4737
Rabbit Polyclonal to Mouse PECAM-1 / CD31
Mouse, Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-PECAM-1 / CD31 Antibody (aa18-590) LS-C150165
Goat Polyclonal (IgG) to Mouse PECAM-1 / CD31
Mouse
IHC, Western blot, Flow Cytometry
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone ER-MP12]
Mouse
IHC - Paraffin, IHC - Frozen
Biotin Conjugated

Details

PECAM-1 / CD31
Rat
Mouse (tested or 100% immunogen sequence identity)
Monoclonal [ER-MP12]
Biotin
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (1:40)
  • IHC - Frozen (1:1600)

Specificity and Use

PECAM-1 / CD31 antibody was raised against murine macrophage precursor cells
The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions) identified as CD31. The antigen expression closely follows that of CD34. Antigen distribution: Isolated cells: The antigen is found on the surface of approximately 40% of freshly isolated bone marrow cells of adult mice, on the majority of immortalized macrophage precursor cell lines (M1, RMB-1, RMB-3) corresponding to CFU-GM cells. The antibody detects pluri- and multipotent stem cells and prothymocytes as well as particular lymphoid cells in bone marrow and peripheral lymphoid organs. Tissue sections: The antigen is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express the antigen. Specificity: Mouse: Major population of colony forming unit macrophage (CFU-M) precursor cells, subpopulation of pre CFU-M and monoblasts, precursor cells of granulocytes. Other: unknown
The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant. For IHC with paraffin embedded sections antigen retrieval with Proteinase K is recommended. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS pH 7.2, 5mg/ml BSA, 0.05% Kathon
500 µl Distilled Water.
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About PECAM-1 / CD31

P16284 NM_000442 NP_000433.4

PECAM1 Antibody, CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PECA1 Antibody, PECAM-1 Antibody, GPIIA Antibody

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes.

Requested From: United States
Date Requested: 12/4/2016

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