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Anti-PECAM-1 / CD31 Antibody (clone 2F7B2) IHC-plus™ LS-B2364

Note: This antibody replaces LS-C47219
Catalog Size Price
LS-B2364-50 50 µg (1 mg/ml) Unavailable

Most Popular PECAM-1 / CD31 Antibodies

Anti-PECAM-1 / CD31 Antibody (clone TLD-3A12) LS-C43483
Mouse Monoclonal [clone TLD-3A12] (IgG1) to Rat PECAM-1 / CD31
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IHC - Frozen, Western blot, Flow Cytometry, ELISA
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Immunohistochemistry Image
Anti-PECAM-1 / CD31 Antibody (clone 2F7B2) IHC-plus™ LS-B2364
Mouse Monoclonal [clone 2F7B2] (IgG1) to Human PECAM-1 / CD31
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PECAM-1 / CD31 Antibody IHC-plus™ LS-B4737
Rabbit Polyclonal to Mouse PECAM-1 / CD31
Mouse, Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-PECAM-1 / CD31 Antibody (aa18-590) LS-C150165
Goat Polyclonal (IgG) to Mouse PECAM-1 / CD31
Mouse
IHC, Western blot, Flow Cytometry
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Immunocytochemistry Image
Anti-PECAM-1 / CD31 Antibody (clone 1H6) IHC-plus™ LS-B10502
Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31
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IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone 2F7B2] (IgG1) to Human PECAM-1 / CD31
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PECAM-1 / CD31
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2F7B2]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

PECAM-1 / CD31 antibody was raised against recombinant human PECAM1 / CD31.
Ni-NTA purified recombinant CD31 expressed in E. coli strain BL21 (DE3).
Immunohistochemistry: LS-B2364 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2364 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, 0.02% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PECAM-1 / CD31

P16284 NM_000442 NP_000433.4

PECAM1 Antibody, CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PECA1 Antibody, PECAM-1 Antibody, GPIIA Antibody

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes.

Immunohistochemistry

Immunohistochemistry
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2364 concentration 5 ug/ml.

Requested From: 
Date Requested: 12/15/2017

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