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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PECAM‑1 / CD31 Antibody (clone 1D2‑1A5) IHC‑plus™ LS‑B5414
Note: This antibody replaces LS-C133373
CD31 antibody LS-B5414 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5414-50
50 µg (1 mg/ml)
Unavailable

Popular PECAM-1 / CD31 Products

IHC of rat spleen cryosection with Mouse anti-Rat CD31 (LS-C43483).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2364 concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4737 concentration 10 ug/ml.
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Anti-CD31 antibody IHC of human tonsil, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5577 dilution 1:100.
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Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10502 concentration 20 ug/ml.
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Product Description

CD31 antibody LS-B5414 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About PECAM-1 / CD31
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. P16284 NM_000442 NP_000433.4

PECAM1 Antibody, CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PECA1 Antibody, PECAM-1 Antibody, GPIIA Antibody


Specifications

Target
Human PECAM-1 / CD31
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1D2-1A5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PECAM-1 / CD31 antibody was raised against pECAM1 (AAH22512, 29 a.a. ~ 739 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PECAM-1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: using tissue lysates, transfected cell lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.

Western blot

PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.
PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.

Western blot

PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.
PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.

Western blot

PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.
PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.

Immunohistochemistry

Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5414 concentration 5 ug/ml.

Western blot

PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.
PECAM1 monoclonal antibody clone 1D2-1A5. Western blot of PECAM1 expression in human placenta.


Requested From: 
Date Requested: 6/20/2018

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