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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PEBP1 / RKIP Antibody (C‑Terminus) LS‑C17572
RKIP antibody LS-C17572 is an unconjugated mouse monoclonal antibody to human RKIP (PEBP1). Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C17572-100
100 µg
$605

Popular PEBP1 / RKIP Products

Anti-PEBP1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3247 concentration 5 ug/ml.
Species: Human, Monkey, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
PBP monoclonal antibody (M01), clone 2G2-1F1. Western Blot analysis of PEBP1 expression in human kidney.
Species: Human
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Immunofluorescence analysis of HeLa cells using PEBP1 antibody. Blue: DAPI for nuclear staining.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, RNA Immunoprecipitation
Immunofluorescent analysis of RKIP staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

RKIP antibody LS-C17572 is an unconjugated mouse monoclonal antibody to human RKIP (PEBP1). Validated for IP and WB.

Specifications

Target
Human PEBP1 / RKIP
Synonyms
PEBP1, HCNP, HCNPpp, PBP, Prostatic binding protein, Raf kinase inhibitor protein, Raf kinase inhibitory protein, Prostatic-binding protein, Neuropolypeptide h3, PEBP, PEBP-1, RKIP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
PEBP1 / RKIP antibody was raised against synthetic peptide derived from the C-terminal region of human RKIP protein.
Epitope
C-Terminus
Specificity
Specific for the C-terminal region of the RKIP protein. On Western blots, it identifies a band at 30kD in RKIP-transfected HEK293 lysates and 23kD in HEK293 lysates or total mouse brain lysates. Reactivity has been confirmed with HEK 293 lysates. Immunoprecipitation tests with HEK293 cell preparations also yielded the ~23kD band.
Usage
Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 7-10 ug/IP reaction. Western Blot: 1-3 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About PEBP1 / RKIP
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase By similarity. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. P30086 NM_002567 NP_002558.1


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Requested From: United States
Date Requested: 10/21/2018

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