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Anti-PEA3 / ETV4 Antibody (aa181-230) IHC-plus™ LS-B1527

Note: This antibody replaces LS-C29900
Catalog Size Price
LS-B1527-50 50 µg (1 mg/ml) Unavailable

Related Products

17 PEA3 / ETV4 Antibodies

Most Popular PEA3 / ETV4 Antibodies

Anti-PEA3 / ETV4 Antibody (aa181-230) IHC-plus™ LS-B1527
Rabbit Polyclonal (IgG) to Human PEA3 / ETV4
Human, Mouse, Rat, Bovine, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-ETV4 / PEA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1527 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PEA3 / ETV4 Antibody (aa419-447) LS-C98380
Rabbit Polyclonal to Human PEA3 / ETV4
Human, Mouse
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-PEA3 / ETV4 Antibody (clone 1A2G3) IHC-plus™ LS-B3895
Mouse Monoclonal [clone 1A2G3] (IgG1) to Human PEA3 / ETV4
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-ETV4 / PEA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3895 dilution 1:200.
Anti-PEA3 / ETV4 Antibody LS-C335603
Rabbit Polyclonal (IgG) to Human PEA3 / ETV4
Human, Mouse
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of U20S cells.
Anti-PEA3 / ETV4 Antibody LS-C665432
Rabbit Polyclonal (IgG) to Human PEA3 / ETV4
Human
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed Publications (1)
Rabbit Polyclonal (IgG) to Human PEA3 / ETV4
Human, Mouse, Rat, Bovine, Pig
IHC, IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PEA3 / ETV4
Rabbit
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Monkey, Bat, Horse, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PEA3 / ETV4 antibody was raised against synthetic peptide located between aa181-230 of human ETV4 (P43268, NP_001977). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Pig (100%); Marmoset, Bat, Rabbit, Horse (92%).
LS-E11278 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1527. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa181-230
Human ETV4 / PEA3
Immunohistochemistry: LS-B1527 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1527 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
Inactivation of Capicua drives cancer metastasis. Okimoto RA, Breitenbuecher F, Olivas VR, Wu W, Gini B, Hofree M, Asthana S, Hrustanovic G, Flanagan J, Tulpule A, Blakely CM, Haringsma HJ, Simmons AD, Gowen K, Suh J, Miller VA, Ali S, Schuler M, Bivona TG. Nature genetics. 2017 49:87-96. (WB; Mouse, Human) [Full Text Article] [PubMed:27869830] [PMC:PMC5195898]

About PEA3 / ETV4

P43268 AF095890 AAD09186.1

ETV4 Antibody, ETS translocation variant 4 Antibody, E1A-F Antibody, E1AF Antibody, Ets variant 4 Antibody, PEA3 Antibody, Protein PEA3 Antibody, PEAS3 Antibody

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

Immunohistochemistry

Anti-ETV4 / PEA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1527 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ETV4 / PEA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1527 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ETV4 / PEA3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1527 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ETV4 / PEA3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1527 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ETV4 / PEA3 antibody LS-B1527 Western blot of Fetal small intestine lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ETV4 / PEA3 antibody LS-B1527 Western blot of Fetal small intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal Small intestine Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal Small intestine Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/24/2018

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