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Anti-PDX1 Antibody (N-Terminus) LS-C110227

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular PDX1 Antibodies

Anti-PDX1 Antibody (aa198-213) IHC-plus™ LS-B3086
Rabbit Polyclonal (IgG) to Human PDX1
Human, Monkey, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PDX1 Antibody (phospho-Thr11) LS-C97137
Rabbit Polyclonal to Human PDX1
Human
Dot Blot
Unconjugated
Dot Blot Image
Anti-PDX1 Antibody (clone 2A12) LS-C114333
Mouse Monoclonal [clone 2A12] (IgG1) to Human PDX1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PDX1
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PDX1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

PDX1 antibody was raised against synthetic peptide from N-Terminus of human PDX1 (P52945, NP_000200). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig (100%); Galago, Panda, Bat, Opossum (92%).
LS-E13615 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human PDX1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PDX1

P52945 NM_000209 NP_000200.1

PDX1 Antibody, Glucose-sensitive factor Antibody, Insulin upstream factor 1 Antibody, IPF1 Antibody, Insulin promoter factor 1 Antibody, IPF-1 Antibody, IUF-1 Antibody, STF-1 Antibody, GSF Antibody, IDX-1 Antibody, Islet/duodenum homeobox-1 Antibody, IUF1 Antibody, MODY4 Antibody, PDX-1 Antibody

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.

Western blot

Western blot
PDX1 antibody LS-C110227 Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/30/2017

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