Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human PDPN / Podoplanin
IHC - Paraffin, IHC - Frozen
Human PDPN / Podoplanin
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin (0.75 - 2 µg/ml)
IHC - Frozen (0.75 - 2 µg/ml)
Specificity and Use
PDPN / Podoplanin antibody was raised against oRF Human podoplanin ectodomain expressed in E. coli.
Recognizes human Podoplanin (gp36).
Suitable for use in Immunohistochemistry (frozen, paraffin). Immunohistochemistry (frozen, paraffin): 0.75-2 ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
PBS, 0.09% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport.