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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDPK1 / PDK1 Antibody (phospho‑Ser/Thr) LS‑C9552 Best Seller
PDK1 antibody LS-C9552 is an unconjugated mouse monoclonal antibody to PDK1 (PDPK1). Validated for ELISA, IP and WB.
Antibody
Catalog
Size
Price
LS-C9552-100
100 µl
$575

Popular PDPK1 / PDK1 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Detection of Human and Mouse PDK1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PDK1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Species: Human, Mouse, Guinea pig
Applications: Western blot, Immunoprecipitation
Western blot of Pdk-1 / PDPK1 antibody LS-C48430.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

PDK1 antibody LS-C9552 is an unconjugated mouse monoclonal antibody to PDK1 (PDPK1). Validated for ELISA, IP and WB.

Specifications

Target
PDPK1 / PDK1
Synonyms
PDPK1, HPDK1, PkB kinase like gene 1, PkB-like 1, Pdk-1, PkB kinase, PRO0461
Host
Mouse
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:20)
  • ELISA (1:2000)
Immunogen
PDPK1 / PDK1 antibody was raised against synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones (KLH). Motif: (FK)XX(F/Y)(S*/T*)(F/Y).
Epitope
pSer/Thr
Specificity
Detects phosphorylated Ser or Thr that is surrounded by Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. It also recognizes peptides containing Lys instead of Phe at the -4 position. Does not cross-react with the non-phosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:20. ELISA (Peptide): 1:1000.
Presentation
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.2% sodium azide, 50% glycerol.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About PDPK1 / PDK1
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. O15530 NM_002613 NP_002604.1


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Requested From: United States
Date Requested: 10/16/2018

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