- Western blot (1:1000)
- Immunoprecipitation (1:20)
- ELISA (1:2000)
PDPK1 / PDK1 antibody was raised against synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones (KLH). Motif: (FK)XX(F/Y)(S*/T*)(F/Y).
Detects phosphorylated Ser or Thr that is surrounded by Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. It also recognizes peptides containing Lys instead of Phe at the -4 position. Does not cross-react with the non-phosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:20. ELISA (Peptide): 1:1000.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.2% sodium azide, 50% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.