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Anti-PDK4 Antibody (aa382-410) IHC-plus™ LS-B3459

Note: This antibody replaces LS-C98959


Wt. Vol. Conc. Price
- 200 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PDK4 Antibodies

Anti-PDK4 Antibody (N-Terminus) LS-C47013
Rabbit Polyclonal to Mouse PDK4
Mouse, Human, Rat
Western blot, ELISA
Anti-PDK4 Antibody (aa382-410) IHC-plus™ LS-B3459
Rabbit Polyclonal to Human PDK4
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-PDK4 Antibody LS-C137183
Rabbit Polyclonal (IgG) to Human PDK4
Human, Mouse, Rat
IHC, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal to Human PDK4
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot


Human PDK4
Human, Mouse (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation


  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)

Specificity and Use

PDK4 antibody was raised against kLH-conjugated synthetic peptide between amino acids 382-410 from C-terminal region of human PDK4.
LS-E8632 - Lyophilized - 100 µg - $145.00
Immunohistochemistry: LS-B3459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3459 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use with mouse in IHC-P.


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (3)

Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression. Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA. The Journal of biological chemistry. 2006 281:39897-906. (WB, ChrIP; Rat) [PubMed:17079227]
Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha). Attia RR, Connnaughton S, Boone LR, Wang F, Elam MB, Ness GC, Cook GA, Park EA. The Journal of biological chemistry. 2010 285:2375-85. [PubMed:19948729] [PMC:PMC2807296]
Coenzyme Q10 reverses mitochondrial dysfunction in atorvastatin-treated mice and increases exercise endurance. Muraki A, Miyashita K, Mitsuishi M, Tamaki M, Tanaka K, Itoh H. Journal of applied physiology (Bethesda, Md. : 1985). 2013 Aug;113:479-86. [Full Text Article] [PubMed:22653988]

About PDK4

Q16654 NM_002612 NP_002603.1

PDK4 Antibody, PDHK4 Antibody

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate.


Anti-PDK4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3459 concentration 10 ug/ml.


Anti-PDK4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3459 concentration 10 ug/ml.


Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.

Western blot

Western blot
PDK4 Antibody (C396) western blot of K562 cell line lysates (35 ug/lane). The PDK4 antibody detected the PDK4 protein (arrow).

Western blot

Western blot
The PDK4 Antibody is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.

Requested From: United States
Date Requested: 10/26/2016

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