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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDK3 Antibody LS‑C163492
PDK3 antibody LS-C163492 is an unconjugated rabbit polyclonal antibody to human PDK3. Validated for Flow and WB.
Catalog
Size
Price
LS-C163492-400
400 µl
Unavailable

Popular PDK3 Products

Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Applications: Western blot
PDK3 antibody LS-C111083 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Western blot
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Applications: IHC, Western blot, ELISA
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Applications: IHC, Western blot, ELISA

Product Description

PDK3 antibody LS-C163492 is an unconjugated rabbit polyclonal antibody to human PDK3. Validated for Flow and WB.
About PDK3
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. Q15120 NM_005391 NP_005382.1

PDK3 Antibody, PDHK3 Antibody


Specifications

Target
Human PDK3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Western blot

Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.

Flow Cytometry

PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.

Flow Cytometry

PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.

Flow Cytometry

PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.

Flow Cytometry

PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK3 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/20/2018

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