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Anti-PDK3 Antibody (aa74-123) LS-C80835

Catalog Size Price
LS-C80835-50 50 µg (1 mg/ml) Unavailable

Related Products

50 PDK3 Antibodies

Most Popular PDK3 Antibodies

Anti-PDK3 Antibody (aa74-123) LS-C80835
Rabbit Polyclonal (IgG) to Human PDK3
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Western blot
Unconjugated
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK3 Antibody (C-Terminus) LS-C111083
Rabbit Polyclonal to Human PDK3
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Chicken, Zebrafish
Western blot
Unconjugated
PDK3 antibody LS-C111083 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK3 Antibody LS-C163492
Rabbit Polyclonal to Human PDK3
Human
Western blot, Flow Cytometry
Unconjugated
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Anti-PDK3 Antibody (Biotin) LS-C687891
Rabbit Polyclonal to Mouse PDK3
Mouse
IHC, Western blot
Biotin Conjugated
Western Blot; Sample: Recombinant protein.
Anti-PDK3 Antibody (FITC) LS-C694793
Rabbit Polyclonal to Mouse PDK3
Mouse
IHC, Western blot
FITC Conjugated
Western Blot; Sample: Recombinant protein.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human PDK3
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PDK3
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

PDK3 antibody was raised against synthetic peptide located between aa74-123 of human PDK3 (Q15120, NP_005382). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%); Turkey, Zebra finch, Chicken, Lizard (92%); Opossum (85%); Guinea pig, Catfish (84%).
aa74-123
Human PDK3
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About PDK3

Q15120 NM_005391 NP_005382.1

PDK3 Antibody, PDHK3 Antibody

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH.

Western blot

Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/27/2018

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