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Anti-PDK2 Antibody (clone 5F8) IHC-plus™ LS-B5413

Note: This antibody replaces LS-C133371
Catalog Size Price
LS-B5413-50 50 µg (0.5 mg/ml) Unavailable

Related Products

59 PDK2 Antibodies

Most Popular PDK2 Antibodies

Anti-PDK2 Antibody (aa260-309) LS-C120281
Rabbit Polyclonal (IgG) to Human PDK2
Human, Mouse, Rat
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Western blot of extracts from HepG2 cells, using PDK2 Antibody. The lane on the right is treated with the synthesized peptide.
Anti-PDK2 Antibody (aa155-379, Biotin) LS-C687887
Rabbit Polyclonal (IgG) to Mouse PDK2
Mouse
IHC, Western blot
Biotin Conjugated
Western blot of recombinant PDK2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK2 Antibody (aa155-379, FITC) LS-C694790
Rabbit Polyclonal (IgG) to Mouse PDK2
Mouse
IHC, Western blot
FITC Conjugated
Western blot of recombinant PDK2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK2 Antibody (aa155-379, Cy3) LS-C703488
Rabbit Polyclonal (IgG) to Mouse PDK2
Mouse
IHC, Western blot
Cy3 Conjugated
Western blot of recombinant PDK2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK2 Antibody (aa155-379, HRP) LS-C712513
Rabbit Polyclonal (IgG) to Mouse PDK2
Mouse
IHC, Western blot
HRP Conjugated
Western blot of recombinant PDK2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Mouse Monoclonal [clone 5F8] (IgG2a,k) to Human PDK2
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PDK2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5F8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

PDK2 antibody was raised against pDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PDK2
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About PDK2

Q15119 NM_002611 NP_002602.2

PDK2 Antibody, PDHK2 Antibody, PDHKII Antibody

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism.

Immunohistochemistry

Anti-PDK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5413 concentration 5 ug/ml.
Anti-PDK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5413 concentration 5 ug/ml.

Western blot

PDK2 monoclonal antibody clone 5F8. Western blot of PDK2 expression in 293.
PDK2 monoclonal antibody clone 5F8. Western blot of PDK2 expression in 293.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PDK2 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK2 is approximately 0.03 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/23/2018

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