Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression. Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA. The Journal of biological chemistry. 2006 281:39897-906. (WB, ChrIP; Rat)
Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure. Richards JG, Sardella BA, Schulte PM. American journal of physiology. Regulatory, integrative and comparative physiology. 2008 295:R979-90.
Effects of environmental hypoxia on cardiac energy metabolism and performance in tilapia. Speers-Roesch B, Sandblom E, Lau GY, Farrell AP, Richards JG. American journal of physiology. Regulatory, integrative and comparative physiology. 2010 298:R104-19.
The relationship between human skeletal muscle pyruvate dehydrogenase phosphatase activity and muscle aerobic capacity. Love LK, LeBlanc PJ, Inglis JG, Bradley NS, Choptiany J, Heigenhauser GJ, Peters SJ. Journal of applied physiology (Bethesda, Md. : 1985). 2011 111:427-34.
Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PDK2 Antibody in mouse intestine tissue lysate (35 ug/lane). PDK2 (arrow) was detected using the purified antibody.
PDK2 Antibody (C392) western blot of HeLa cell line lysates (35 ug/lane). The PDK2 antibody detected the PDK2 protein (arrow).
Western blot of PDK2 (arrow) using PDK2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).