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Catalog Number Size Price
LS-C331622-20 20 µl $287 
LS-C331622-50 50 µl $341 
LS-C331622-100 100 µl $431 
LS-C331622-200 200 µl $539 
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse testis using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded human kidney using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunofluorescence analysis of U2OS cells using PDPK1 antibody. Blue: DAPI for nuclear staining.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of various cell lines, using PDPK1 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of various cell lines, using PDPK1 antibody.
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse testis using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded human kidney using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDPK1 Antibodyat dilution of 1:200 (40x lens).
PDPK1 / PDK1 Antibody - Immunofluorescence analysis of U2OS cells using PDPK1 antibody. Blue: DAPI for nuclear staining.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of various cell lines, using PDPK1 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of various cell lines, using PDPK1 antibody.
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Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (IHC, IF, WB) LS‑C331622

Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (IHC, IF, WB) LS‑C331622

Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C331622-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PDK1 antibody LS-C331622 is an unconjugated rabbit polyclonal antibody to PDK1 (PDPK1) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
Target
Human PDPK1 / PDK1
Synonyms
PDPK1 | HPDK1 | PkB kinase like gene 1 | PkB-like 1 | Pdk-1 | PkB kinase | PRO0461
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1 (NP_112558.2). AGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
Specificity
Human PDK1 / PDPK1
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 48kDa/50kDa/58kDa/60kDa/63kDa, while the observed MW by Western blot was 65kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDPK1 / PDK1
O15530 NM_002613 NP_002604.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024